Restriction Enzymes
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Filtered Search Results
Thermo Scientific™ FastDigest BpiI (IIs class)
Cut at GAAGAC(2/6)^ sites with Thermo Scientific™ FastDigest™ BbsI (BpiI), which performs best using universal FastDigest Buffer (Isoschizomers: BpuAI, BstV2I).
Thermo Scientific™ FastDigest PstI
The FastDigest PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BspMAI).
| Sensitive to Heat Inactivation | No |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Pst I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 3' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Product Line | FastDigest |
Thermo Scientific™ FastDigest ApaI
Cut at GGGCC^C sites with Thermo Scientific™ FastDigest™ ApaI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PspOMI).
Thermo Scientific™ FastDigest Value Pack
FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in a single buffer.
Thermo Scientific™ FastDigest SspI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ GTP Solution (100 mM)
Thermo Scientific GTP (guanosine 5'-triphosphate) is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.
Thermo Scientific™ HindIII (10 U/μL)
The HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer.
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ FastDigest KpnI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest NdeI
The FastDigest NdeI restriction enzyme recognizes CA^TATG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: FauNDI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Nde I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 65°C |
| Product Line | FastDigest |
Thermo Scientific™ FastDigest PacI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ Reaction Buffer with MgCl2 for DNase I (10X)
Optimize reaction condions for RNase-free DNase I with this 10X reaction Tris-HCl (pH 7.5) buffer containing MgCl2.
Thermo Scientific™ FastDigest ScaI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ Cfr9I (XmaI) (10 U/μL)
The Cfr9I (XmaI) restriction enzyme recognizes C^CCGGG sites and cuts best at 37°C in its own unique buffer (Isoschizomers: TspMI, XmaCI, XmaI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Cfr9I (XmaI) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | Unique Buffer (10X Buffer Cfr9I (XmaI)) |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ T4 DNA Ligase Buffer (10X)
Safely ligate samples with this buffer, for use with T4 DNA Ligase and tested for the absence of endo- and exodeoxyribonucleases and ribonucleases.
Thermo Scientific™ FastDigest KflI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.